Inferring subunit stoichiometry from single molecule photobleaching

@article{Hines2013InferringSS,
  title={Inferring subunit stoichiometry from single molecule photobleaching},
  author={Keegan Hines},
  journal={The Journal of General Physiology},
  year={2013},
  volume={141},
  pages={737 - 746}
}
  • Keegan Hines
  • Published 2013
  • Medicine, Chemistry
  • The Journal of General Physiology
  • Single molecule photobleaching is a powerful tool for determining the stoichiometry of protein complexes. By attaching fluorophores to proteins of interest, the number of associated subunits in a complex can be deduced by imaging single molecules and counting fluorophore photobleaching steps. Because some bleaching steps might be unobserved, the ensemble of steps will be binomially distributed. In this work, it is shown that inferring the true composition of a complex from such data is… CONTINUE READING

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