Inferring gene regulatory logic from high-throughput measurements of thousands of systematically designed promoters

@inproceedings{Sharon2012InferringGR,
  title={Inferring gene regulatory logic from high-throughput measurements of thousands of systematically designed promoters},
  author={Eilon Sharon and Yael Kalma and A. D. L. Sharp and Tali Raveh-Sadka and Michal Levo and Danny Zeevi and Leeat Keren and Zohar Yakhini and Adina Weinberger and Eran Segal},
  booktitle={Nature Biotechnology},
  year={2012}
}
Despite extensive research, our understanding of the rules according to which cis-regulatory sequences are converted into gene expression is limited. We devised a method for obtaining parallel, highly accurate gene expression measurements from thousands of designed promoters and applied it to measure the effect of systematic changes in the location, number, orientation, affinity and organization of transcription-factor binding sites and nucleosome-disfavoring sequences. Our analyses reveal a… CONTINUE READING
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