Inferring Genetic Networks and Identifying Compound Mode of Action via Expression Profiling

@article{Gardner2003InferringGN,
  title={Inferring Genetic Networks and Identifying Compound Mode of Action via Expression Profiling},
  author={Timothy S. Gardner and Diego di Bernardo and David Lorenz and James J. Collins},
  journal={Science},
  year={2003},
  volume={301},
  pages={102 - 105}
}
The complexity of cellular gene, protein, and metabolite networks can hinder attempts to elucidate their structure and function. To address this problem, we used systematic transcriptional perturbations to construct a first-order model of regulatory interactions in a nine-gene subnetwork of the SOS pathway in Escherichia coli. The model correctly identified the major regulatory genes and the transcriptional targets of mitomycin C activity in the subnetwork. This approach, which is… 
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