Infectivity and neutralization of Cryptosporidium parvum sporozoites.

  title={Infectivity and neutralization of Cryptosporidium parvum sporozoites.},
  author={Michael W. Riggs and Lance E. Perryman},
  journal={Infection and immunity},
  volume={55 9},
Cryptosporidiosis, a diarrheal disease of calves and humans caused by the coccidian parasite Cryptosporidium parvum, is terminated in hosts with normal immune systems. To assess the mechanisms of immunity in cryptosporidiosis, it is necessary to isolate and quantitate sporozoites, the infective stage of Cryptosporidium spp. Here we report the (i) separation of infective C. parvum oocysts from calf feces by ether extraction, sieving, and hypochlorite treatment; (ii) separation of viable C… CONTINUE READING