Infectious cDNA clones of two grapevine viruses

  title={Infectious cDNA clones of two grapevine viruses},
  author={Pasquale Saldarelli and Mariangela Dell’Orco and Angelantonio Minafra},
  journal={Archives of Virology},
 Full-length cDNA copies of the genomes of Grapevine virus A (GVA) and Grapevine virus B (GVB) under the control of bacteriophage T7 promoter have been synthesized, which were refractory to cloning in Escherichia coli. However, both transcribed cDNAs were infectious when mechanically inoculated to Nicotiana plants. A full-length cDNA copy of GVB was engineered in pCass2, a plasmid containing a partially duplicated copy of the Ca35S promoter, but was rather unstable in Escherichia coli. No… CONTINUE READING


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Expression of the coat protein genes of grapevine virus A and B in Nicotianaspecies and evaluation of the resistance conferred on transgenic plants

  • A Minafra, R Golles, +6 authors M Laimer da Camara Machado
  • J Plant Pathol
  • 1998
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