Infectious RNA transcribed from an engineered full-length cDNA template of the genome of a pestivirus.

@article{Moormann1996InfectiousRT,
  title={Infectious RNA transcribed from an engineered full-length cDNA template of the genome of a pestivirus.},
  author={Rob J. M. Moormann and H. G. P. van Gennip and G. K. W. Miedema and Marcel Hulst and Piet A van Rijn},
  journal={Journal of virology},
  year={1996},
  volume={70 2},
  pages={
          763-70
        }
}
Infectious RNA was transcribed for the first time from a full-length cDNA template of the plus-strand RNA genome of a pestivirus. The genome of the C strain, which is a vaccine strain of classical swine fever virus, was sequenced and used to synthesize the template. The cDNA sequence of the C strain was found to be 12,311 nucleotides in length and contained one large open reading frame encoding a polyprotein of 3,898 amino acids. Although there were mostly only small differences between the… CONTINUE READING

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