Infection of tobacco mesophyll protoplasts by tobacco mosaic virus.

  title={Infection of tobacco mesophyll protoplasts by tobacco mosaic virus.},
  author={Itaru Takebe and Yoshiaki Otsuki},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={64 3},
It was proved that the protoplasts prepared from mesophyll of Nicotiana tabacum are infected by tobacco mosaic virus. The infection occurred when purified tobacco mosaic virus particles were added to a protoplast suspension in the presence of poly-L-ornithine. The virus multiplied in these protoplasts to a level of 10(6) virus particles per infected protoplast during 24 hours of incubation. The efficiency of infection was remarkably high, exceeding that by mechanical inoculation of tobacco… CONTINUE READING