Infection of human cells by an endogenous retrovirus of pigs

@article{Patience1997InfectionOH,
  title={Infection of human cells by an endogenous retrovirus of pigs},
  author={Clive Patience and Y. Takeuchi and Robin A. Weiss},
  journal={Nature Medicine},
  year={1997},
  volume={3},
  pages={282-286}
}
The possible use of pig organs and tissues as xenografts in humans is actively being considered in biomedical research. We therefore examined whether pig endogenous retrovirus (PERV) genomes can be infectiously transmitted to human cells in culture. Two pig kidney cell lines spontaneously produce C-type retrovirus particles. Cell-free retrovirus produced by the PK-15 kidney cell line (PERV-PK) infected pig, mink and human kidney 293 cell lines and co-cultivation of X-irradiated PK-15 cells with… Expand
Expression of pig endogenous retrovirus by primary porcine endothelial cells and infection of human cells
TLDR
Analysis of pig primary aortic endothelial cells together with other transplantation-relevant porcine cells and tissues for expression of PERV mRNA suggests a serious risk of retrovirus transfer after xenotransplantation. Expand
Productive infection of human primary cells and cell lines with porcine endogenous retroviruses.
TLDR
First clinical transplantations of pig cells into humans or ex vivo perfusions did not result in transmission of PERVs, and infections with PERV in vivo were shown for human peripheral blood mononuclear cells, primary endothelial cells, and primary aortic smooth muscle cells. Expand
Porcine endogenous retroviruses: in vitro host range and attempts to establish small animal models.
TLDR
In vitro infectivity was demonstrated for permanent cell lines and primary cells from a wide range of species and no antibodies against PERV and no provirus integration were observed in any of the treated animals, suggesting that productive infection of these animals did not occur. Expand
Characterization of Clones of Human Cell Line Infected with Porcine Endogenous Retrovirus (PERV) from Porcine Cell Line, PK-15
TLDR
The sequences of PERVs were detected in human cell clones after PERV infection, but PERV virions could not be detected from the culture supernatant by RT assay. Expand
Type C Retrovirus Released from Porcine Primary Peripheral Blood Mononuclear Cells Infects Human Cells
TLDR
It is shown here that mitogenic activation of peripheral blood mononuclear cells isolated from the National Institutes of Health miniature pig and the Yucatan pig resulted in the activation and release of an infectious type C retrovirus. Expand
Transmissible infection of human 293T cells with porcine endogenous retroviruses subgroup a from NIH-miniature pig.
TLDR
The results suggested that a prevention study of PERV xenotransmission from experimental miniature pigs should concentrate on PERV-A control, as all subgroups of infectious PERV virion were detected in the primary cell culture media. Expand
Microchimerism and transmission of porcine endogenous retrovirus from a pig cell line or specific pathogen-free pig islets to mouse tissues and human cells during xenografts in nude mice
TLDR
Microchimerism and PERV transmission were frequently observed in both mouse and human tissues during grafting of pig PK15 cells into nude mice bearing human tumours, and sometimes during pig islet xenograft in this model. Expand
Porcine endogenous retroviruses inhibit human immune cell function: risk for xenotransplantation?
TLDR
It is reported that purified PERV particles show a protein pattern typical for type C retroviruses and are antigenically related to mammalian leukemia viruses, indicating that high titer replication of PERVs in the transplant recipient could therefore lead to an immunodeficiency disease. Expand
No Evidence of the Productive Replication of Porcine Endogenous Retrovirus (PERV) from SNU Miniature Pigs in Human Cell Line
TLDR
In vitro infectivity test suggests that PERV from SNU miniature pig might not replicate productively in human cell lines although it could infect human cells and integrate into chromosome. Expand
Packaging of human endogenous retrovirus sequences is undetectable in porcine endogenous retrovirus particles produced from human cells.
TLDR
The results indicate that the potential for recombination of PERV and HERV sequences is low and that novel viruses generated by this mechanism are unlikely to represent a significant risk for xenotransplantation. Expand
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