Infection of Cell Lines with Experimental and Natural Ovine Scrapie Agents

  title={Infection of Cell Lines with Experimental and Natural Ovine Scrapie Agents},
  author={Michael H. Neale and Susan J Mountjoy and Jane C. Edwards and Didier Vilette and Hubert Laude and Otto Windl and Ginny C. Saunders},
  journal={Journal of Virology},
  pages={2444 - 2452}
ABSTRACT Mouse bioassay remains the gold standard for determining proof of infectivity, strain type, and infectious titer estimation in prion disease research. The development of an approach using ex vivo cell-based assays remains an attractive alternative, both in order to reduce the use of mice and to hasten results. The main limitation of a cell-based approach is the scarcity of cell lines permissive to infection with natural transmissible spongiform encephalopathy strains. This study… 
A Bovine Cell Line That Can Be Infected by Natural Sheep Scrapie Prions
It is believed that PES cells used together with other prion permissive cell lines will prove a valuable tool for ongoing efforts to understand and defeat prions and prion diseases.
Susceptibility of GT1–7 cells to mouse-passaged field scrapie isolates with a long incubation
Characterization of the mechanisms of the specific susceptibility of GT1–7 cells to isolates with a long incubation time may lead to a greater understanding of the differences among prion strains.
Estimating Chronic Wasting Disease infectivity in cell culture
This is the first report demonstrating that a transgenic PrP overexpressing cell line could be infected with a primary inoculate derived from CWD-infected cervids.
A transfectant RK13 cell line permissive to classical caprine scrapie prion propagation
It is suggested that caprine scrapie prions can be propagated in cpRK13 cells, thus this cell line may be a useful tool for the assessment of classical caprine prions in the brain tissues of goats.
A Simple, Versatile and Sensitive Cell-Based Assay for Prions from Various Species
It is shown that epithelial RK13, a cell line permissive to mouse and bank vole prion strains and to natural prion agents from sheep and cervids, enables a robust and sensitive detection of mouse and ovine-derived prions.
The Standard Scrapie Cell Assay: Development, Utility and Prospects
An overview on establishing the Standard Scrapie Cell Assay, its use in studies of disease dissemination and pathogenesis, potential pitfalls and a number of remaining challenges are provided.
Expression of Heterologous PrP and Prion Propagation in RK13 Cells.
The generation of stable RK13 cell clones expressing a heterologous PrP protein in an inducible manner, the establishment and maintenance of chronically infected cultures, and the selection of cell clones suitable for cell-based titration of prions are described.
Methods for Differentiating Prion Types in Food-Producing Animals
Prion typing methodologies are reviewed in light of current and emerging prion types in food-producing animals, including bovine spongiform encephalopathy and chronic wasting disease in cervids.


Ex vivo propagation of infectious sheep scrapie agent in heterologous epithelial cells expressing ovine prion protein
Developing a permanent cell line supporting propagation of natural sheep scrapie and showing that cultivated cells from a nonneuronal origin can efficiently replicate prions show that the species barrier can be crossed ex vivo through the expression of a relevant PrP gene.
Successful Transmission of Three Mouse-Adapted Scrapie Strains to Murine Neuroblastoma Cell Lines Overexpressing Wild-Type Mouse Prion Protein
The results corroborate the hypothesis that the successful transmission of agents ex vivo depends on both expression levels of host PrPC and the sequence of PrPSc and provide a basis for the development of highly susceptible cell lines that could be used in diagnostic and therapeutic approaches to the TSEs.
Selection of ovine PrP high-producer subclones from a transfected epithelial cell line.
Assaying prions in cell culture: the standard scrapie cell assay (SSCA) and the scrapie cell assay in end point format (SCEPA).
A cell-based prion assay that is based on the isolation of cell lines highly susceptible to certain strains of mouse prions and a method for identifying individual, prion-infected cells and quantifying them is developed, suitable for robotization.
A cell line infectible by prion strains from different species.
It is shown that mouse and bank vole prions can be propagated in RK13 cells that express the corresponding PrP(C) and the respective molecular patterns of abnormal PrP (PrP(res)) and the neuropathological features of the infecting strains appeared to be maintained during the propagation in cell culture.
Prion propagation in cultured cells.
Infected cell culture models have provided some valuable insights into the biogenesis of PrP(Sc) in terms of conversion, subcellular localisations, physiopathological consequences and species-barrier determinants.
Susceptibility of common fibroblast cell lines to transmissible spongiform encephalopathy agents.
It is demonstrated that the common laboratory fibroblast cell lines NIH/3T3 and L929, which express low levels of cellular mouse prion protein, are susceptible to infection with mouse-adapted scrapie and suggest that any cell line expressing normal host prionprotein could have the potential to support propagation of TSE agents.
Strain characterization of natural sheep scrapie and comparison with BSE.
There is agent strain variation in natural scrapie in sheep and that the spectrum of strains present may have changed over the last 20 years, according to the Sinc or PrP genotype of the mouse used for passage.
Scrapie strain variation and mutation.
  • M. Bruce
  • Biology
    British medical bulletin
  • 1993
Transmissions to mice from natural scrapie and BSE suggest that strain variation exists in the field, and these findings have important implications when considering the molecular nature of the scrapie agent and the details of agent-host interactions.