Infection of Cell Lines with Experimental and Natural Ovine Scrapie Agents

@article{Neale2009InfectionOC,
  title={Infection of Cell Lines with Experimental and Natural Ovine Scrapie Agents},
  author={Michael H. Neale and Susan J Mountjoy and Jane C. Edwards and Didier Vilette and Hubert Laude and Otto Windl and Ginny C. Saunders},
  journal={Journal of Virology},
  year={2009},
  volume={84},
  pages={2444 - 2452}
}
ABSTRACT Mouse bioassay remains the gold standard for determining proof of infectivity, strain type, and infectious titer estimation in prion disease research. The development of an approach using ex vivo cell-based assays remains an attractive alternative, both in order to reduce the use of mice and to hasten results. The main limitation of a cell-based approach is the scarcity of cell lines permissive to infection with natural transmissible spongiform encephalopathy strains. This study… 
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29 Citations
Highly Influential Citations
2
Background Citations
9
Methods Citations
4

29 Citations

A Bovine Cell Line That Can Be Infected by Natural Sheep Scrapie Prions
TLDR
It is believed that PES cells used together with other prion permissive cell lines will prove a valuable tool for ongoing efforts to understand and defeat prions and prion diseases.
Susceptibility of GT1–7 cells to mouse-passaged field scrapie isolates with a long incubation
TLDR
Characterization of the mechanisms of the specific susceptibility of GT1–7 cells to isolates with a long incubation time may lead to a greater understanding of the differences among prion strains.
hTERT-immortalized ovine microglia propagate natural scrapie isolates.
Estimating Chronic Wasting Disease infectivity in cell culture
TLDR
This is the first report demonstrating that a transgenic PrP overexpressing cell line could be infected with a primary inoculate derived from CWD-infected cervids.
A transfectant RK13 cell line permissive to classical caprine scrapie prion propagation
TLDR
It is suggested that caprine scrapie prions can be propagated in cpRK13 cells, thus this cell line may be a useful tool for the assessment of classical caprine prions in the brain tissues of goats.
A Simple, Versatile and Sensitive Cell-Based Assay for Prions from Various Species
TLDR
It is shown that epithelial RK13, a cell line permissive to mouse and bank vole prion strains and to natural prion agents from sheep and cervids, enables a robust and sensitive detection of mouse and ovine-derived prions.
The Standard Scrapie Cell Assay: Development, Utility and Prospects
TLDR
An overview on establishing the Standard Scrapie Cell Assay, its use in studies of disease dissemination and pathogenesis, potential pitfalls and a number of remaining challenges are provided.
Correlation of cellular factors and differential scrapie prion permissiveness in ovine microglia.
Expression of Heterologous PrP and Prion Propagation in RK13 Cells.
TLDR
The generation of stable RK13 cell clones expressing a heterologous PrP protein in an inducible manner, the establishment and maintenance of chronically infected cultures, and the selection of cell clones suitable for cell-based titration of prions are described.
Methods for Differentiating Prion Types in Food-Producing Animals
TLDR
Prion typing methodologies are reviewed in light of current and emerging prion types in food-producing animals, including bovine spongiform encephalopathy and chronic wasting disease in cervids.
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References

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TLDR
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