Inexpensive Multiplexed Library Preparation for Megabase-Sized Genomes

  title={Inexpensive Multiplexed Library Preparation for Megabase-Sized Genomes},
  author={Michael Baym and Sergey Kryazhimskiy and Tami D. Lieberman and Hattie Chung and Michael M Desai and Roy Kishony and Stefan J Green},
  booktitle={PloS one},
Whole-genome sequencing has become an indispensible tool of modern biology. However, the cost of sample preparation relative to the cost of sequencing remains high, especially for small genomes where the former is dominant. Here we present a protocol for rapid and inexpensive preparation of hundreds of multiplexed genomic libraries for Illumina sequencing. By carrying out the Nextera tagmentation reaction in small volumes, replacing costly reagents with cheaper equivalents, and omitting… CONTINUE READING
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