Industrial scale production of plasmid DNA for vaccine and gene therapy: plasmid design, production, and purification

@article{Prather2003IndustrialSP,
  title={Industrial scale production of plasmid DNA for vaccine and gene therapy: plasmid design, production, and purification},
  author={K. Prather and S. Sagar and J. C. Murphy and M. Chartrain},
  journal={Enzyme and Microbial Technology},
  year={2003},
  volume={33},
  pages={865-883}
}
Abstract The past several years have witnessed a rapidly increasing number of reports on utilizing plasmid DNA as a vector for the introduction of genes into mammalian cells for use in both gene therapy and vaccine applications. “Naked DNA vaccines” allow the foreign genes to be transiently expressed in transfected cells, mimicking intracellular pathogenic infection and triggering both the humoral and cellular immune responses. While considerable attention has been paid to the potential of such… Expand
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