Industrial enzyme applications.

  title={Industrial enzyme applications.},
  author={Ole Kirk and Torben Vedel Borchert and Claus Crone Fuglsang},
  journal={Current opinion in biotechnology},
  volume={13 4},

Directed evolution of industrial enzymes: an update.

Directed Evolution: An Approach to Engineer Enzymes

By providing a simple and reliable route to enzyme improvement, directed evolution has emerged as a key technology for enzyme engineering and biocatalysis.

Post-production modification of industrial enzymes

This review will focus on post-production modification of enzymes and its use and opportunities in industry.

Biocatalysts: application and engineering for industrial purposes

The present review seeks to highlight the major fields of enzyme application and to provide an updated overview on previous protein engineering studies wherein natural enzymes were modified to meet the operational conditions required for industrial application.

Commercial Application of Rumen Microbial Enzymes

The enzyme as a biocatalyst is an important tool in various industries where these enzymes have been used in the development of various products and services in the eco-friendly manner. The use of

Recent progress in biocatalyst discovery and optimization.

3.02 – Industrial Enzymes

Immobilized Enzymes from the Class of Oxidoreductases in Technological Processes: A Review

The main fields of application of immobilized enzymes from the class of oxidoreductases and prospects for their use in technological processes were analyzed. The structure of the most important

Fungal Biotechnology for Industrial Enzyme Production: Focus on (Hemi)cellulase Production Strategies, Advances and Challenges

This chapter will discuss the challenges and biotechnological approaches used in development of novel or improved enzymes for industrial applications as well as fungal strains with increased enzyme production and secretion capacity.

Enzymes in Industrial Biotechnology

The detergent industry is the largest user of enzymes, which are utilized to remove soiling, increase softness, and protect garments, and the starch industry uses a variety of enzymes in the production of value-added products from starches.



Directed evolution of new catalytic activity using the α/β-barrel scaffold

It is found that targeting the catalytic template for in vitro mutagenesis and recombination, followed by in vivo selection, results in a new phosphoribosylanthranilate isomerase that has catalytic properties similar to those of the natural enzyme, with an even higher specificity constant.

Industrial biocatalysis today and tomorrow

Biocatalytic processes can now be carried out in organic solvents as well as aqueous environments, so that apolar organic compounds aswell as water-soluble compounds can be modified selectively and efficiently with enzymes and bioc atalytically active cells.

Biotechnological development of effective phytases for mineral nutrition and environmental protection

A synopsis of the concept of "ideal phytase" and the biotechnological approaches for developing such an enzyme is provided and examples of Escherichia coli AppA and Aspergillus fumigatus PhyA are presented to illustrate how new phytases are identified from microorganisms and developed by genetic engineering based on the gene sequences and protein structures of these enzymes.

Optimization of the catalytic properties of Aspergillus fumigatus phytase based on the three‐dimensional structure

This investigation attempted to increase the specific activity of Aspergillus fumigatus phytase with the ultimate goal to combine the most favorable properties of all phytases in a single protein.

Fuel ethanol production from lignocellulose: a challenge for metabolic engineering and process integration

Improvement of the fermentation process is just one of several factor that needs to be fully optimized and integrated to generate a competitive lignocellulose ethanol plant.

Development of Industrially Important α-Amylases

A two amino acid deletion in the region from position 180-184 increased the overall stability both with respect to temperature and calcium sensitivity, to a satisfactory level comparable to that of TermamylTM.


Transglutaminase is an enzyme that forms crosslinks between protein molecules. This crosslinkage has unique effects on protein properties, gelation capability, thermal stability, water-holding

DNA shuffling of subgenomic sequences of subtilisin

DNA family shuffling of 26 protease genes was used to create a library of chimeric proteases that was screened for four distinct enzymatic properties, producing a great diversity of property combinations in the progeny enzymes.