1,248 Citations
Directed Evolution: An Approach to Engineer Enzymes
- BiologyCritical reviews in biotechnology
- 2006
By providing a simple and reliable route to enzyme improvement, directed evolution has emerged as a key technology for enzyme engineering and biocatalysis.
Post-production modification of industrial enzymes
- Biology, ChemistryApplied Microbiology and Biotechnology
- 2014
This review will focus on post-production modification of enzymes and its use and opportunities in industry.
Biocatalysts: application and engineering for industrial purposes
- Engineering, BiologyCritical reviews in biotechnology
- 2016
The present review seeks to highlight the major fields of enzyme application and to provide an updated overview on previous protein engineering studies wherein natural enzymes were modified to meet the operational conditions required for industrial application.
Commercial Application of Rumen Microbial Enzymes
- Engineering, Biology
- 2015
The enzyme as a biocatalyst is an important tool in various industries where these enzymes have been used in the development of various products and services in the eco-friendly manner. The use of…
Recent progress in biocatalyst discovery and optimization.
- Biology, ChemistryCurrent opinion in chemical biology
- 2004
Immobilized Enzymes from the Class of Oxidoreductases in Technological Processes: A Review
- ChemistryCatalysis in Industry
- 2019
The main fields of application of immobilized enzymes from the class of oxidoreductases and prospects for their use in technological processes were analyzed. The structure of the most important…
Fungal Biotechnology for Industrial Enzyme Production: Focus on (Hemi)cellulase Production Strategies, Advances and Challenges
- Biology, Engineering
- 2016
This chapter will discuss the challenges and biotechnological approaches used in development of novel or improved enzymes for industrial applications as well as fungal strains with increased enzyme production and secretion capacity.
Enzymes in Industrial Biotechnology
- Biology
- 2016
The detergent industry is the largest user of enzymes, which are utilized to remove soiling, increase softness, and protect garments, and the starch industry uses a variety of enzymes in the production of value-added products from starches.
References
SHOWING 1-10 OF 56 REFERENCES
Directed evolution of new catalytic activity using the α/β-barrel scaffold
- Biology, ChemistryNature
- 2000
It is found that targeting the catalytic template for in vitro mutagenesis and recombination, followed by in vivo selection, results in a new phosphoribosylanthranilate isomerase that has catalytic properties similar to those of the natural enzyme, with an even higher specificity constant.
Industrial biocatalysis today and tomorrow
- Engineering, BiologyNature
- 2001
Biocatalytic processes can now be carried out in organic solvents as well as aqueous environments, so that apolar organic compounds aswell as water-soluble compounds can be modified selectively and efficiently with enzymes and bioc atalytically active cells.
Optimization of the catalytic properties of Aspergillus fumigatus phytase based on the three‐dimensional structure
- Biology, ChemistryProtein science : a publication of the Protein Society
- 2000
This investigation attempted to increase the specific activity of Aspergillus fumigatus phytase with the ultimate goal to combine the most favorable properties of all phytases in a single protein.
Fuel ethanol production from lignocellulose: a challenge for metabolic engineering and process integration
- EngineeringApplied Microbiology and Biotechnology
- 2001
Improvement of the fermentation process is just one of several factor that needs to be fully optimized and integrated to generate a competitive lignocellulose ethanol plant.
Development of Industrially Important α-Amylases
- Biology
- 1999
A two amino acid deletion in the region from position 180-184 increased the overall stability both with respect to temperature and calcium sensitivity, to a satisfactory level comparable to that of TermamylTM.
TRANSGLUTAMINASE: ITS UTILIZATION IN THE FOOD INDUSTRY
- Chemistry
- 2001
Transglutaminase is an enzyme that forms crosslinks between protein molecules. This crosslinkage has unique effects on protein properties, gelation capability, thermal stability, water-holding…
Glucose isomerase: insights into protein engineering for increased thermostability.
- Biology, ChemistryBiochimica et biophysica acta
- 2000
DNA shuffling of subgenomic sequences of subtilisin
- BiologyNature Biotechnology
- 1999
DNA family shuffling of 26 protease genes was used to create a library of chimeric proteases that was screened for four distinct enzymatic properties, producing a great diversity of property combinations in the progeny enzymes.
Directed evolution: the 'rational' basis for 'irrational' design.
- BiologyCurrent opinion in structural biology
- 2000