Induction of tetraploidy in Buddleia globosa

  title={Induction of tetraploidy in Buddleia globosa},
  author={Judith B. Rose and J. Kubba and Ken R. Tobutt},
  journal={Plant Cell, Tissue and Organ Culture},
The aim was to produce a tetraploid form of Buddleia globosa to facilitate introgression of yellow flower colour into B. davidii, which is naturally tetraploid. Protocols were established for the micropropagation of B. globosa and tetraploid plants were obtained by application in vitro of colchicine to pre-cultured excised nodal sections. Three concentrations of colchicine were applied (0.01%, 0.05% and 0.1% w/v) for 1, 2 or 3 days. At 0.01% tetraploids were produced only after 2 days of… CONTINUE READING

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