Induction of sulfadiazine resistance in vitro in Toxoplasma gondii.

  title={Induction of sulfadiazine resistance in vitro in Toxoplasma gondii.},
  author={Christelle Doliwa and Sandie Escotte-Binet and Dominique Aubert and Fr{\'e}d{\'e}ric Velard and Aline Schmid and R{\'e}gine Geers and Isabella Villena},
  journal={Experimental parasitology},
  volume={133 2},
We induced sulfadiazine resistance in two sulfadiazine sensitive strains of Toxoplasma gondii, RH (Type I) and ME-49 (Type II) in vitro by using drug pressure. At first, sulfadiazine susceptibility of the two sensitive strains and two naturally resistant strains of T. gondii was evaluated on Vero cells using an enzyme-linked immunosorbent assay (ELISA). The IC(50) values of sulfadiazine were 77 μg/mL for RH, 51 μg/mL for ME-49 and higher than 1000 μg/mL for the two natural resistant strains… CONTINUE READING