Induction of somatic mutation in a human B cell line in vitro.

  title={Induction of somatic mutation in a human B cell line in vitro.},
  author={S Den{\'e}poux and Diane Razanajaona and Dominique Blanchard and G Meffre and J. Donald Capra and Jacques Banchereau and Serge J. Lebecque},
  volume={6 1},
Both the B cell-surface trigger(s) and the intracellular molecular mechanism(s) of somatic hypermutation in immunoglobulin (Ig) variable region genes remain unknown, partly because of the lack of a simple and reproducible in vitro model. Here, we show that upon surface immunoglobulin cross-linking followed by co-culture with activated cloned T cells, the Burkitt's lymphoma cell line BL2 is induced to mutate its IgV(H) gene. Repeated activation of BL2 cells increased the frequency of mutation… CONTINUE READING

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