Induction of senescence-associated genes by 5-bromodeoxyuridine in HeLa cells.

  title={Induction of senescence-associated genes by 5-bromodeoxyuridine in HeLa cells.},
  author={Toshikazu Suzuki and Sachi Minagawa and Eriko Michishita and Hideki Ogino and Michihiko Fujii and Youji Mitsui and Dai Ayusawa},
  journal={Experimental gerontology},
  volume={36 3},
5-Bromodeoxyuridine (BrdU) universally induces a senescence-like phenomenon in mammalian cells. To assess this phenomenon at the level of gene expression, we constructed a PCR-based subtractive cDNA library enriched for mRNA species that immediately increase by administration of BrdU to HeLa cells. Candidate cDNA clones were isolated by differential colony hybridization, and then positive clones were identified by Northern blot analysis. Sequencing analysis revealed that the identified cDNA… CONTINUE READING

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