Induction of protein-protein interactions in live cells using light

  title={Induction of protein-protein interactions in live cells using light},
  author={Masayuki Yazawa and Amir Masoud Sadaghiani and Brian Hsueh and Ricardo E Dolmetsch},
  journal={Nature Biotechnology},
Protein-protein interactions are essential for many cellular processes. We have developed a technology called light-activated dimerization (LAD) to artificially induce protein hetero- and homodimerization in live cells using light. Using the FKF1 and GIGANTEA (GI) proteins of Arabidopsis thaliana, we have generated protein tags whose interaction is controlled by blue light. We demonstrated the utility of this system with LAD constructs that can recruit the small G-protein Rac1 to the plasma… CONTINUE READING
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