Induction of protein-protein interactions in live cells using light

@article{Yazawa2009InductionOP,
  title={Induction of protein-protein interactions in live cells using light},
  author={M. Yazawa and A. M. Sadaghiani and Brian Hsueh and R. Dolmetsch},
  journal={Nature Biotechnology},
  year={2009},
  volume={27},
  pages={941-945}
}
Protein-protein interactions are essential for many cellular processes. We have developed a technology called light-activated dimerization (LAD) to artificially induce protein hetero- and homodimerization in live cells using light. Using the FKF1 and GIGANTEA (GI) proteins of Arabidopsis thaliana, we have generated protein tags whose interaction is controlled by blue light. We demonstrated the utility of this system with LAD constructs that can recruit the small G-protein Rac1 to the plasma… Expand

Paper Mentions

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TLDR
This chapter reviews the different properties of light-induced dimerization systems, based on plant phytochromes, cryptochromes, and light-oxygen-voltage domain proteins, exploring advantages and limitations of the different systems and practical considerations related to their use. Expand
Control of Protein Function through Optochemical Translocation
TLDR
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