Induction of prophage lambda by γ-rays, mitomycin C and tif; Repressor cleavage studied by immunoprecipitation

@article{Shinagawa1977InductionOP,
  title={Induction of prophage lambda by γ-rays, mitomycin C and tif; Repressor cleavage studied by immunoprecipitation},
  author={Hideo Shinagawa and Kiyoshi Mizuuchi and Peter T. Emmerson},
  journal={Molecular and General Genetics MGG},
  year={1977},
  volume={155},
  pages={87-91}
}
The fate of the λ repressor on induction of E. coli lysogens has been studied after γ-irradiation, mitomycin C treatment or by temperature step-up of a tif-1 mutant. The repressor and a fragment of the repressor were selectively precipitated from labelled cell lysates by anti-repressor serum and subjected to gel electrophoresis and autoradiography. Induction of lysogens by all of these treatments leads to cleavage of the repressor. Repressor cleavage closely parallels the inactivation of its… CONTINUE READING
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