Induction of polyamine limitation in Chinese hamster ovary cells by alpha-methylornithine.


Chinese hamster ovary (CHO) cells in culture were limited for polyamines through the use of alpha-methylornithine (alpha MO), a competitive inhibitor of ornithine decarboxylase. Initial exposure of the cells to the inhibitor caused growth rate and intracellular polyamine content to decline continuously. Reseeding the alpha MO-treated cells into medium… (More)