Induction of nitric oxide synthase in glial cells.

  title={Induction of nitric oxide synthase in glial cells.},
  author={Miles Simmons and Sean Murphy},
  journal={Journal of neurochemistry},
  volume={59 3},
Primary astrocyte cultures, C6 glioma cells, and N18 neuroblastoma cells were assayed for nitric oxide synthase (NOS) activity with a bioassay of cyclic GMP production in RFL-6 fibroblasts. Treatment of astrocyte cultures for 16-18 h with lipopolysaccharide (LPS) induced NOS-like activity that was L-arginine and NADPH dependent, Ca2+ independent, and potentiated by superoxide dismutase. Induction was evident after 4 h, was dependent on the dose of LPS, and required protein synthesis. Treatment… CONTINUE READING