Induction of microspore embryogenesis inBrassica napus L. by gamma irradiation and ethanol stress

  title={Induction of microspore embryogenesis inBrassica napus L. by gamma irradiation and ethanol stress},
  author={Paul M. Pechan and Wilfred A. Keller},
  journal={In Vitro Cellular & Developmental Biology},
Gamma irradiation and ethanol stress treatments redirected pollen development to an embryo formation pathway inBrassica napus. Less than 0.01% of microspores developed into embryos at 25°C compared to approximately 2% at 32°C. However, subsequent to gamma irradiation and ethanol treatments up to 1% and 0.7% of microspores formed embryos at 25°C, respectively. Gamma irradiation also enhanced embryogenesis at 32°C. The possible importance of these findings is discussed in relation to microspore… CONTINUE READING
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Publications referenced by this paper.
Showing 1-10 of 12 references

An efficient method for culture of isolated microspores in Brassica napus

W. A. Keller, Z. Fan, P. M Pechan
Proc. 7th International Rapeseed Congress • 1988
View 1 Excerpt

Identification of potentially embryogenic microspores in Brassica napus

P. M. Pechan, W. A. Keller
Physiol. Plant • 1988
View 1 Excerpt

The enhancement of anther culture efficiency in Brassica napus ssp. oleifera Metzg (Sinsk) using low doses of gamma irradiation

M. V. MacDonald, M. A. Hadwiger, F. N Aslam
New Phytol • 1988

The effect of X-irradiation and formaldehyde treatment of spermatogonia on the reversion of an unstable, P-element insertion mutation in Drosophila melanogaster

J.C.J. Eeken, F. H. Sobels
Mutation Res • 1986
View 1 Excerpt