Induction of micronuclei in mouse bone marrow after combined X‐rays‐cyclophosphamide and X‐rays‐mitomycin C treatments

  title={Induction of micronuclei in mouse bone marrow after combined X‐rays‐cyclophosphamide and X‐rays‐mitomycin C treatments},
  author={Małgorzata Dobrzyńska and Antoni K. Gajewski},
  journal={Teratogenesis Carcinogenesis and Mutagenesis},
: The induction of micronuclei in polychromatic erythrocytes of bone marrow of Pzh:SWISS mice after combined treatment with X-rays and cyclophosphamide (CP) or X-rays and mitomycin C (MMC) were investigated. Combinations of high (1.00 Gy + 100 mg/kg bw CP and 1. 00 Gy + 5.25 mg/kg bw MMC) and low (0.25 Gy + 25 mg/kg bw CP and 0. 25 Gy + 1.75 mg/kg bw MMC) doses were used. Both chemicals enhanced the mutagenic effects caused by irradiation. After combined treatment with high doses of X-rays + CP… Expand
Micronucleus formation induced by the combination of low doses of X-rays and antineoplastic drugs in bone marrow of male mice.
  • M. Dobrzyńska
  • Chemistry, Medicine
  • Teratogenesis, carcinogenesis, and mutagenesis
  • 2000
The effects of combined exposures of nonmutagenic doses of X-rays and anticancer agents (cyclophosphamide, mitomycin C, and vinblastine) have been investigated on the induction of micronuclei in the bone marrow of laboratory mice. Expand
The effects in mice of combined treatments to X-rays and antineoplastic drugs in the Comet assay.
Strong increases of DNA damage in bone marrow lymphocytes and in germ cells were observed by comparison with the results obtained for each agent acting alone, but effects were observed over a similar dose range. Expand
Assessment of DNA damage in multiple organs from mice exposed to X-rays or acrylamide or a combination of both using the comet assay.
Combined exposure to X-rays and AA enhanced DNA damage after single exposure to each agent. Expand
In vivo Evaluation of the Genotoxic Effects of Clomiphene Citrate on Rat Reticulocytes: A Micronucleus Genotoxicity
In rats, the micronucleus genotoxicity assay suggests a dose-dependent CC effect on genomic instability in bone marrow stem cells in vivo, and this effect is associated with genotoxic effects on reticulocytes in vivo. Expand
In vivo evaluation of the genotoxic effects of gonadotropins on rat reticulocytes.
In rats, the micronucleus genotoxicity assay suggests a dose-dependent gonadotropin effect on genomic instability in bone marrow stem cells in vivo, which is similar to that seen in women given ovulation-inducing drugs. Expand


Mouse dominant lethal and sperm abnormality studies with combined exposure to X-rays and mitomycin C.
The induction of dominant lethal effects and sperm abnormalities in Pzh:Swiss male mice after treatments with X-rays and mitomycin C (MMC) was investigated and MMC increased the frequency of abnormal spermatozoa after exposure alone and in combination withX-rays. Expand
Evaluation of spermatogenic response of mice to the induction of mutations by combined treatment with X rays and antineoplastic drugs
Enhanced risk could be shown not only after high but also after low combined exposure to X-ray plus cyclophosphamide and X-rays plus mitomycin C, and with low doses this enhanced risk was observed in spermatids for X- Ray exposure and in differentiating s permatogonia to early sperMatocytes for X/ray exposure. Expand
Factors affecting the induction of micronuclei at low doses of X-rays, MMS and dimethylnitrosamine in mouse erythroblasts.
In erythrocytes from mouse bone marrow the time schedule of micronucleus formation in relation to the last DNA synthesis was investigate by [3H]thymidine labelling in the autoradiographic technique.Expand
Dose response at low doses of X-irradiation and MMS on the induction of micronuclei in mouse erythroblasts.
The test of induced micronuclei in erythrocytes of mammalian bone marrow constitutes a suitable method for the screening of induced chromosomal lesions at very low dosages of chemicals or irradiations, and suggests that X-irradiation does not interfere with the repair process operating with MMS. Expand
Comparison of single and multiple treatment regimens in the mouse bone marrow micronucleus assay for hydroquinone (HQ) and cyclophosphamide (CP).
The present data suggest that the response of the bone marrow micronucleus assay to multiple treatments may depend on the dose employed and may differ from chemical to chemical. Expand
Relationship between experimental results in mammals and man. I. Cytogenetic analysis of bone marrow injury induced by a single dose of cyclophosphamide.
The presented results tend to a conclusion that micronucleus testing may be a very suitable method used for screening purpose, however, the method of classical cytogenetic analysis, especially the evaluation of breaks, still remains the most exact and reliable technique. Expand
Mouse bone-marrow response to low doses of whole-body gamma irradiation: induction of micronuclei.
The induction of micronuclei in mouse bone marrow was studied after whole-body exposure to low doses of gamma radiation, ranging from 5 to 75 cGy, and a linear dose response was obtained at both postirradiation times. Expand
Potentiation by caffeine of the frequencies of micronuclei induced by mitomycin C and cyclophosphamide in young mice.
The pregnancy had no effect on MMC- or CP-induced clastogenicity although a tendency to a decreased sensitivity to the damaging activity of MMC seemed to be detected in pregnant C57Bl mice compared to virgin female animals. Expand
Micronucleus test and bone-marrow chromosome analysis: a comparison of 2 methods in vivo for evaluating chemically induced chromosomal alterations.
The sensitivities of 2 cytogenetic tests, chromosome analysis and the micronucleus test, were compared by using mice exposed to the substances methyl methanesulfonate (MMS), mitomycin C (MC) andExpand
A comparison of the incidence of micronuclei in blood and bone marrow in 3 strains of mouse dosed with cyclophosphamide or hexamethylphosphoramide (HMPA).
Significant increases in micronuclei were seen in circulating erythrocytes only at 48 h in C57Bl/6J mice with both test chemicals and in C3H/C57 mice only with cyclophosphamide. Expand