Induction of metallothionein and other mRNA species by carcinogens and tumor promoters in primary human skin fibroblasts.

@article{Angel1986InductionOM,
  title={Induction of metallothionein and other mRNA species by carcinogens and tumor promoters in primary human skin fibroblasts.},
  author={Peter Angel and A. Poeting and Ujjal K Mallick and Hans Jobst Rahmsdorf and Michael Schorpp and Peter A. Herrlich},
  journal={Molecular and cellular biology},
  year={1986},
  volume={6 5},
  pages={1760-6}
}
We used nucleic acid hybridization and cDNA cloning techniques to isolate human sequences that respond to the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). These clones were used as probes to examine changes of gene expression that occurred after the proliferation of exponentially growing primary human fibroblasts was arrested. Transcript levels detected by these probes were increased coordinately by treatment of the cells with UV light, mitomycin C, TPA, or the UV light-induced… CONTINUE READING