Induction of increased permeability of polarized enterocyte monolayers by enterotoxigenic Escherichia coli heat-labile enterotoxin.

@article{Kreisberg2011InductionOI,
  title={Induction of increased permeability of polarized enterocyte monolayers by enterotoxigenic Escherichia coli heat-labile enterotoxin.},
  author={Roderick B Kreisberg and Jill Harper and Maura C Strauman and Mark E. Marohn and John S. Clements and James P Nataro},
  journal={The American journal of tropical medicine and hygiene},
  year={2011},
  volume={84 3},
  pages={451-5}
}
Enterotoxigenic Escherichia coli (ETEC) is a common cause of acute diarrhea in resource-poor settings. We report that some ETEC strains elicit a reduction in trans-epithelial electrical resistance (TER) in polarized T84 epithelial cell monolayers. The effect was irreversible up to 48 hours after a three-hour infection and was observed with heat-labile enterotoxin (LT)-producing strains, but not with heat-stable enterotoxin (ST)-producing strains. Using purified LT, a mutant with reduced ADP… CONTINUE READING