Induction of immediate early genes by cyclic AMP in primary cultures of neurons from rat cerebral cortex.

@article{Vaccarino1993InductionOI,
  title={Induction of immediate early genes by cyclic AMP in primary cultures of neurons from rat cerebral cortex.},
  author={Flora M Vaccarino and Mike D. Hayward and Helen N Le and D J Hartigan and Ronald S Duman and Eric J Nestler},
  journal={Brain research. Molecular brain research},
  year={1993},
  volume={19 1-2},
  pages={76-82}
}
In this paper, we tested whether physiological activators of the cAMP second messenger pathway in primary cultures of neurons from rat cerebral cortex directly induce c-fos and other immediate early gene (IEG) transcription factors. We have found that brief (30 s to 2 min) stimulation of neurons with vasoactive intestinal peptide (VIP) and SKF-38393, a D1-dopaminergic receptor agonist, potently increased mRNA levels for the IEGs c-fos, jun-B, and NGFI-A, with weaker increases for c-jun. This… CONTINUE READING