Induction of human airway smooth muscle apoptosis by neutrophils and neutrophil elastase.

  title={Induction of human airway smooth muscle apoptosis by neutrophils and neutrophil elastase.},
  author={Ute Oltmanns and Maria B. Sukkar and Shaoping Xie and M. John and Kian Fan Chung},
  journal={American journal of respiratory cell and molecular biology},
  volume={32 4},
  • U. Oltmanns, M. Sukkar, K. Chung
  • Published 14 January 2005
  • Biology, Medicine
  • American journal of respiratory cell and molecular biology
Neutrophils are an important component of airway inflammation and may interact with human airway smooth muscle cells (HASMC). We investigated the effect of neutrophils and of neutrophil-derived proteases on HASMC survival. When co-incubated with neutrophils (0.1-1 x 10(6) cells/ml), attachment of human ASMC was reduced to 12.3 +/- 4.3% compared with untreated controls after 72 h. HASMC showed nuclear condensation and fragmentation (41.6 +/- 8.1% compared with baseline of 3.1 +/- 0.4%), and the… 
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