Induction of fertile male flowers in genetically female Cannabis sativa plants by silver nitrate and silver thiosulphate anionic complex

  title={Induction of fertile male flowers in genetically female Cannabis sativa plants by silver nitrate and silver thiosulphate anionic complex},
  author={H. Y. Mohan Ram and Rina Sett},
  journal={Theoretical and Applied Genetics},
Key ResultSummaryApical application of silver nitrate (AgNO3; 50 and 100 μg per plant) and silver thiosulphate anionic complex (Ag(S2O3)23−; STS; 25, 50 and 100 μg per plant) to female plants of Cannabis sativa induced the formation of reduced male, intersexual and fully altered male flowers on the newly formed primary lateral branches (PLBs); 10 μg per plant of AgNO3 was ineffective and 150 μg treatment proved inhibitory.

Foliar Sprays of Silver Thiosulfate Produce Male Flowers on Female Hemp Plants

It is demonstrated that male flowers can be produced easily and consistently on female plants through application of foliar sprays of STS under short-day conditions.

Pollen Appearance and In Vitro Germination Varies for Five Strains of Female Hemp Masculinized Using Silver Thiosulfate

Genetically female seed is sought for cannabidiol (CBD) hemp production because CBD is extracted from the flowers of female hemp plants. The production of all female seed requires masculinization of

Induction of fertile flowers in potato (Solanum tuberosum L.) by silver thiosulphate anionic complex

Silver thiosulphate in combination with extended photoperiod effectively induced flowering in 16 potato genotypes studied for flowering induction and enhanced maximum flowering and duration of flowering to a great extent in the normally flowering genotypes.

Silver thiosulfate and Benzyladenine in combination with pruning additively feminizes cassava flowers and modulates transcriptome

It is concluded that treatments with pruning, STS and BA create widespread changes on the network of hormone signaling and regulatory factors beyond ethylene and cytokinin to improve flower production and fruit set in cassava.

Hermaphroditism in Marijuana (Cannabis sativa L.) Inflorescences – Impact on Floral Morphology, Seed Formation, Progeny Sex Ratios, and Genetic Variation

The morphological features of anthers, pollen production and germination in hermaphroditic flowers and in staminate inflorescences on male plants were compared and Copia-like retrotransposons within the C. sativa genome which may be associated with the expression of male or female phenotype were revealed.

Production of Feminized Seeds of High CBD Cannabis sativa L. by Manipulation of Sex Expression and Its Application to Breeding

This is the first comprehensive report on the induction of fertile male flowers on female plants from dioecious medical cannabis and it showed that spraying whole plants with STS once is more efficient than the application of STS on shoot tips while spraying plants with 0.01% GA and intensive cutting is ineffective in stimulating the production of male flowers.

Flower Development in Cassava Is Feminized by Cytokinin, While Proliferation Is Stimulated by Anti-Ethylene and Pruning: Transcriptome Responses

It is concluded that flower-enhancing treatments with pruning, STS, and BA create widespread changes in the network of hormone signaling and regulatory factors beyond ethylene and cytokinin.

Microgametophyte Development in Cannabis sativa L. and First Androgenesis Induction Through Microspore Embryogenesis

This represents the first report concerning androgenesis induction in C. sativa, which lays the foundations for double haploid research in this species.

Fertilization Following Pollination Predominantly Decreases Phytocannabinoids Accumulation and Alters the Accumulation of Terpenoids in Cannabis Inflorescences

Fertilization substantially decreased the concentration of nearly all phytocannabinoids in the plant regardless of the type of fertilizing male, and may point to the functional roles of secondary metabolites in Cannabis.

Induction and Morphological Characterization of Hermaphrodite Flowers in a Gynoecious Line of Bitter Gourd by Silver Nitrate, Gibberellic Acid, and Silver Thiosulfate

Silver nitrate applied at 6 mm was best for inducing hermaphrodite sex form in the gynoecious line of bitter gourd, which had ovaries, stigma, styles, stamens, anthers, calyx, corolla, and petioles that were double the size of normal female flowers.



Induction of male flowers in a pistillate line ofRicinus communis L. by silver and cobalt ions

Aqueous solutions of silver nitrate and cobalt chloride injected into the main stem of plants of the pistillate cv.

Ethylene: A Natural Regulator of Sex Expression of Cucumis melo L.

It is proposed that ethylene is an endogenous regulator of sex expression in C. sativus and C. melo, a gynoecious sex type that normally produce only pistillate (female) flowers, and when grown with hypobaric ventilation to facilitate removal of endogenous gases by diffusion, produced perfect flowers.

Silver Ion: A Potent Antiethylene Agent in Cucumber and Tomato1

Ag+, applied foliarly as AgNO3 to intact cucumber (Cucumis sativus L. cv. Wisconsin SMR-18 and GY3) and tomato (Lycopersicon esculentum cv. Homestead) plants, was a potent inhibitor of ethylene

Comparative effect of silver ion and gibberellic acid on the induction of male flowers on femaleCannabis plants

Silver ion applied as AgNO3 to the shoot tip of female plants ofCannabis induces male flowers. It is more effective than gibberellic acid (GA3) in maintaining the induced state.

A potent inhibitor of ethylene action in plants.

  • E. Beyer
  • Biology, Medicine
    Plant physiology
  • 1976
Ag(I), applied foliarly as AgNO(3), effectively blocked the ability of exogenously applied ethylene to elicit the classical "triple" response in intact etiolated peas and should prove useful in defining further the role of ethylene in plant growth.

Development of Pistillate Castor

The breeding and developmental behaviour of 240 pistillate indicates it to be of the NES type and the expression of a few interspersed male flowers is not confined to any particular order of racemes but is temperature dependent.

Pulse Treatments with the Silver Thiosulfate Complex Extend the Vase Life of Cut Carnations1

Immersing stems of carnation flowers (Dianthus caryophyllus L. cv. White Sim) in solutions containing a silver thiosulfate complex prepared by combining silver nitrate with sodium thiosulfate (molar