Induction of fertile male flowers in genetically female Cannabis sativa plants by silver nitrate and silver thiosulphate anionic complex

  title={Induction of fertile male flowers in genetically female Cannabis sativa plants by silver nitrate and silver thiosulphate anionic complex},
  author={H. Y. Mohan Ram and Rina Sett},
  journal={Theoretical and Applied Genetics},
Key ResultSummaryApical application of silver nitrate (AgNO3; 50 and 100 μg per plant) and silver thiosulphate anionic complex (Ag(S2O3)23−; STS; 25, 50 and 100 μg per plant) to female plants of Cannabis sativa induced the formation of reduced male, intersexual and fully altered male flowers on the newly formed primary lateral branches (PLBs); 10 μg per plant of AgNO3 was ineffective and 150 μg treatment proved inhibitory.Expand
Foliar Sprays of Silver Thiosulfate Produce Male Flowers on Female Hemp Plants
It is demonstrated that male flowers can be produced easily and consistently on female plants through application of foliar sprays of STS under short-day conditions. Expand
Pollen Appearance and In Vitro Germination Varies for Five Strains of Female Hemp Masculinized Using Silver Thiosulfate
Genetically female seed is sought for cannabidiol (CBD) hemp production because CBD is extracted from the flowers of female hemp plants. The production of all female seed requires masculinization ofExpand
Induction of fertile flowers in potato (Solanum tuberosum L.) by silver thiosulphate anionic complex
Silver thiosulphate in combination with extended photoperiod effectively induced flowering in 16 potato genotypes studied for flowering induction and enhanced maximum flowering and duration of flowering to a great extent in the normally flowering genotypes. Expand
Silver thiosulfate and Benzyladenine in combination with pruning additively feminizes cassava flowers and modulates transcriptome
It is concluded that treatments with pruning, STS and BA create widespread changes on the network of hormone signaling and regulatory factors beyond ethylene and cytokinin to improve flower production and fruit set in cassava. Expand
Hermaphroditism in Marijuana (Cannabis sativa L.) Inflorescences – Impact on Floral Morphology, Seed Formation, Progeny Sex Ratios, and Genetic Variation
The morphological features of anthers, pollen production and germination in hermaphroditic flowers and in staminate inflorescences on male plants were compared and Copia-like retrotransposons within the C. sativa genome which may be associated with the expression of male or female phenotype were revealed. Expand
Flower Development in Cassava Is Feminized by Cytokinin, While Proliferation Is Stimulated by Anti-Ethylene and Pruning: Transcriptome Responses
It is concluded that flower-enhancing treatments with pruning, STS, and BA create widespread changes in the network of hormone signaling and regulatory factors beyond ethylene and cytokinin. Expand
Microgametophyte Development in Cannabis sativa L. and First Androgenesis Induction Through Microspore Embryogenesis
This represents the first report concerning androgenesis induction in C. sativa, which lays the foundations for double haploid research in this species. Expand
Induction and Morphological Characterization of Hermaphrodite Flowers in a Gynoecious Line of Bitter Gourd by Silver Nitrate, Gibberellic Acid, and Silver Thiosulfate
Silver nitrate applied at 6 mm was best for inducing hermaphrodite sex form in the gynoecious line of bitter gourd, which had ovaries, stigma, styles, stamens, anthers, calyx, corolla, and petioles that were double the size of normal female flowers. Expand
Comparative RNA-Seq analysis reveals genes associated with masculinization in female Cannabis sativa
Using RNA profiling, several silver thiosulfate-induced genes that potentially control the masculinization of female Cannabis sativa plants are identified and would be useful for understanding predisposed plants producing flowers of both sex types in the same plant, and help breeders to regulate the masculinity of female plants through targeted breeding and plant biotechnology. Expand
Dioecious hemp (Cannabis sativa L.) plants do not express significant sexually dimorphic morphology in the seedling stage
Some economically important crop species are dioecious, producing pollen and ovules on distinct, unisexual, individuals. On-the-spot diagnosis of sex is important to breeders and farmers for cropExpand


Induction of male flowers in a pistillate line ofRicinus communis L. by silver and cobalt ions
Aqueous solutions of silver nitrate and cobalt chloride injected into the main stem of plants of the pistillate cv. Expand
Ethylene: A Natural Regulator of Sex Expression of Cucumis melo L.
It is proposed that ethylene is an endogenous regulator of sex expression in C. sativus and C. melo, a gynoecious sex type that normally produce only pistillate (female) flowers, and when grown with hypobaric ventilation to facilitate removal of endogenous gases by diffusion, produced perfect flowers. Expand
Comparative effect of silver ion and gibberellic acid on the induction of male flowers on femaleCannabis plants
Silver ion applied as AgNO3 to the shoot tip of female plants ofCannabis induces male flowers. It is more effective than gibberellic acid (GA3) in maintaining the induced state.
A potent inhibitor of ethylene action in plants.
  • E. Beyer
  • Biology, Medicine
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Ag(I), applied foliarly as AgNO(3), effectively blocked the ability of exogenously applied ethylene to elicit the classical "triple" response in intact etiolated peas and should prove useful in defining further the role of ethylene in plant growth. Expand
The effect of silver thiosulphate preservative on the physiology of cut carnations I. Influence on longevity and carbohydrate status
The post-harvest life of cut carnation flowers was increased dramatically when the cut stems were immersed in silver thiosulphate for ten miutes, and interference with carbohydrate transport was correlated with «sleepiness» and senescence of the petals. Expand
Development of Pistillate Castor
The breeding and developmental behaviour of 240 pistillate indicates it to be of the NES type and the expression of a few interspersed male flowers is not confined to any particular order of racemes but is temperature dependent. Expand
Mobility and ionic form of silver as related to longevity of cut carnations
A significant improvement of the longevity of carnation flowers in the presence or absence of ethephon is shown, even after a short treatment with the silverthiosulphate complex, possibly associated with the antiethylene action. Expand
Effects of silver on ethylene synthesis and action in cut carnations
  • H. Veen
  • Medicine, Chemistry
  • Planta
  • 2004
Silver, applied as silverthiosulphate, completely blocked the ethylene surge preceding the wilting of the petals and caused the flowers to become insensitive to an ethylene treatment, extending vase life by nearly 100%. Expand
Hormones and flower sex
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Pulse treatment with silver thiosul - phate complex extends the vase life of cut carnations
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