Induction of eIF-4E phosphorylation by the addition of L-pyrroline-5-carboxylic acid to rabbit reticulocyte lysate.

@article{Mick1988InductionOE,
  title={Induction of eIF-4E phosphorylation by the addition of L-pyrroline-5-carboxylic acid to rabbit reticulocyte lysate.},
  author={Sybille Mick and R D Abramson and B. K. Ray and William C. Merrick and R E Thach and Curt H. Hagedorn},
  journal={FEBS letters},
  year={1988},
  volume={236 2},
  pages={484-8}
}
Addition of L-pyrroline-5-carboxylic acid to reticulocyte lysates inhibits protein synthesis and induced phosphoproteins of 25 and 14 kDa. The 25 kDa phosphoprotein had the same Mr and pI as phosphorylated eIF-4E. Incubation of lysates with L-pyrroline-5-carboxylic acid did not alter the crosslinking of eIF-4E to reovirus mRNA caps. These results suggest… CONTINUE READING