Induction of bacterial mercury- and copper-responsive promoters: Functional differences between inducible systems and implications for their use in gene-fusions for in vivo metal biosensors

We have compared the induction by the cognate metal salts of two promoters responsible for metal-resistance gene expression in bacteria. The mercuric ion resistance promoter, P merTPAD , of transposon Tn501 and the copper resistance promoter, P pcoE , from plasmid pRJ1004 were separately cloned to express thelacZ gene under the regulation of their… CONTINUE READING