Induction of apoptotic DNA fragmentation and c-jun downregulation in human myeloid leukemia cells by the permeant Ca2+ chelator BAPTA/AM.

@article{Grant1995InductionOA,
  title={Induction of apoptotic DNA fragmentation and c-jun downregulation in human myeloid leukemia cells by the permeant Ca2+ chelator BAPTA/AM.},
  author={Steven Grant and Alex J. Freemerman and Peter Colin Gregory and H. Jose Antonio Martin and Anthony J. Turner and Ross B. Mikkelsen and Jeya Chelliah and Saul Yanovich and W. David Jarvis},
  journal={Oncology research},
  year={1995},
  volume={7 7-8},
  pages={
          381-92
        }
}
The permeant Ca2+ chelator acetoxymethyl-1,2-bis(2-aminopheoxy)ethane- N,N,N',N'-tetraacetic acid (BAPTA/AM), an agent previously used to characterize drug-induced apoptosis in neoplastic cells, has been examined with respect to induction of DNA fragmentation and cytotoxicity in the human leukemia cell lines HL-60 and U937. Exposure of cells to various concentrations of BAPTA/AM for 6 h resulted in a biphasic induction of internucleosomal DNA cleavage, with maximal damage occurring at 10-microM… CONTINUE READING