Induction of antigen-specific CD8+ T cells, T helper cells, and protective levels of antibody in humans by particle-mediated administration of a hepatitis B virus DNA vaccine.

@article{Roy2000InductionOA,
  title={Induction of antigen-specific CD8+ T cells, T helper cells, and protective levels of antibody in humans by particle-mediated administration of a hepatitis B virus DNA vaccine.},
  author={Mike J Roy and Mary S Wu and Lori J Barr and James T. Fuller and Lynda G Tussey and Sylvia Speller and J. Jacob Culp and Joseph K. Burkholder and William F. Swain and Ruth M Dixon and Georg Widera and Rupert S J Vessey and Abigail King and G Ogg and Awen Gallimore and Joel R. Haynes and D Heydenburg Fuller},
  journal={Vaccine},
  year={2000},
  volume={19 7-8},
  pages={764-78}
}
A DNA vaccine against the hepatitis B virus (HBV) was evaluated for safety and induction of immune responses in 12 healthy, hepatitis-naïve human volunteers using the needle-free PowderJect system to deliver gold particles coated with DNA directly into cells of the skin. Three groups of four volunteers received three administrations of DNA encoding the surface antigen of HBV at one of the three dose levels (1, 2, or 4 microg). The vaccine was safe and well tolerated, causing only transient and… CONTINUE READING