Induction of an anionic peroxidase in cowpea leaves by exogenous salicylic acid.

@article{Fernandes2006InductionOA,
  title={Induction of an anionic peroxidase in cowpea leaves by exogenous salicylic acid.},
  author={Cl{\'e}berson F Fernandes and Vadjah C P Moraes and I. M. M. Vasconcelos and Joaquim Alben{\'i}sio Gomes da Silveira and Jose T A Oliveira},
  journal={Journal of plant physiology},
  year={2006},
  volume={163 10},
  pages={1040-8}
}
Two isoperoxidases were detected in cowpea (Vigna unguiculata) leaves. Treatment of the primary leaves with 10mM salicylic acid increased the total peroxidase activity contributed by the anionic isoform. To isolate both the anionic and cationic peroxidases the leaf crude extract was loaded on a Superose 12 HR 10/30 column followed by chromatography on Mono-Q HR 5/5. Both enzymes were stable in a pH range from 5 to 7. The optimum-temperatures for the cationic and anionic peroxidase isoforms were… CONTINUE READING

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