Induction of a hemogenic program in mouse fibroblasts.

  title={Induction of a hemogenic program in mouse fibroblasts.},
  author={Carlos-Filipe Pereira and Betty Y. Chang and Jiajing Qiu and Xiaohong Niu and Dmitri A. Papatsenko and Caroline E Hendry and Neil R. Clark and Aya Nomura-Kitabayashi and Jason C Kovacic and Avi Ma'ayan and Christoph Schaniel and Ihor Lemischka and Kateri Moore},
  journal={Cell stem cell},
  volume={13 2},
Definitive hematopoiesis emerges during embryogenesis via an endothelial-to-hematopoietic transition. We attempted to induce this process in mouse fibroblasts by screening a panel of factors for hemogenic activity. We identified a combination of four transcription factors, Gata2, Gfi1b, cFos, and Etv6, that efficiently induces endothelial-like precursor cells, with the subsequent appearance of hematopoietic cells. The precursor cells express a human CD34 reporter, Sca1, and Prominin1 within a… CONTINUE READING