Induction of Pyridine Alkaloid Formation in Transformed Root Cultures of Nicotiana tabacum

  title={Induction of Pyridine Alkaloid Formation in Transformed Root Cultures of Nicotiana tabacum},
  author={Rawia A. Zayed and Michael Wink},
  journal={Zeitschrift f{\"u}r Naturforschung C},
  pages={869 - 874}
  • R. Zayed, M. Wink
  • Published 1 July 2008
  • Chemistry
  • Zeitschrift für Naturforschung C
Hairy root cultures of Nicotiana tabacum were set up by excised root tips with Agrobacterium rhizogenes. The successful transformation was confirmed by analyzing rolC and virC genes using polymerase chain reaction (PCR). Hairy root cultures were employed to study the formation of pyridine alkaloids, mainly nicotine. The transformed cultures were incubated with potential elicitors, such as methyljasmonate, quercetin and salicylic acid, in order to stimulate the biosynthesis of pyridine alkaloids… 
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