Induction of Integral Membrane PAM Expression in AtT-20 Cells Alters the Storage and Trafficking of POMC and PC1

@article{Ciccotosto1999InductionOI,
  title={Induction of Integral Membrane PAM Expression in AtT-20 Cells Alters the Storage and Trafficking of POMC and PC1 },
  author={Giuseppe Donato Ciccotosto and Martin R. Schiller and Betty A Eipper and Richard E Mains},
  journal={The Journal of Cell Biology},
  year={1999},
  volume={144},
  pages={459 - 471}
}
Peptidylglycine alpha-amidating monooxygenase (PAM) is an essential enzyme that catalyzes the COOH-terminal amidation of many neuroendocrine peptides. The bifunctional PAM protein contains an NH2-terminal monooxygenase (PHM) domain followed by a lyase (PAL) domain and a transmembrane domain. The cytosolic tail of PAM interacts with proteins that can affect cytoskeletal organization. A reverse tetracycline-regulated inducible expression system was used to construct an AtT-20 corticotrope cell… CONTINUE READING