Induction of HSP70 in cultured rat neonatal cardiomyocytes by hypoxia and metabolic stress.

@article{Iwaki1993InductionOH,
  title={Induction of HSP70 in cultured rat neonatal cardiomyocytes by hypoxia and metabolic stress.},
  author={Koji Iwaki and Shawn Chi and Wolfgang H. Dillmann and Ruben Mestril},
  journal={Circulation},
  year={1993},
  volume={87 6},
  pages={2023-32}
}
BACKGROUND A cultured neonatal rat cardiomyocyte model is used to investigate the expression of the inducible heat shock protein 70 (HSP70i) during hypoxia/reoxygenation and metabolic stress. METHODS AND RESULTS The major HSP70i is increased in its expression at the mRNA and protein level in myocytes exposed to hypoxia/reoxygenation and metabolic stress by the addition of 2-deoxyglucose and sodium cyanide, which are inhibitors known to block ATP production. Surprisingly, the appearance of… CONTINUE READING