Induction of DNA synthesis in isolated nuclei by cytoplasmic factors: inhibition by protease inhibitors.

@article{Wong1987InductionOD,
  title={Induction of DNA synthesis in isolated nuclei by cytoplasmic factors: inhibition by protease inhibitors.},
  author={Ruth L. Wong and J K Gutowski and Marion Katz and Ronald H. Goldfarb and Sharon R Cohen},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1987},
  volume={84 1},
  pages={241-5}
}
Cytoplasmic extracts from spontaneously proliferating and mitogen-activated lymphoid cells contain a protein factor called ADR (activator of DNA replication) that induces DNA synthesis in isolated quiescent nuclei. ADR-containing preparations have proteolytic activity, as indicated by their ability to degrade fibrin in a plasminogen-independent and plasminogen-dependent manner. In addition, aprotinin, a nonspecific protease inhibitor, abrogates ADR-induced DNA synthesis in a dose-dependent… CONTINUE READING