Induction of DNA strand breaks by ethylene oxide in human diploid fibroblasts.

@article{Nygren1994InductionOD,
  title={Induction of DNA strand breaks by ethylene oxide in human diploid fibroblasts.},
  author={Jonas O Nygren and Bj{\"o}rn E Cedervall and Susanna Eriksson and M{\'a}ria Du{\vs}insk{\'a} and Ada Kolman},
  journal={Environmental and molecular mutagenesis},
  year={1994},
  volume={24 3},
  pages={161-7}
}
In vitro exposure of normal human diploid fibroblasts (strain VH-10) to ethylene oxide (EtO) induced DNA strand breaks in the dose range of 2.5-30 mMh of EtO. Alkaline DNA unwinding (ADU), neutral filter elution (NFE), pulsed field gel electrophoresis (PFGE), and the comet assay were used to measure DNA single (SSBs) and double strand breaks (DSBs… CONTINUE READING