Induction and membrane expression of heat shock proteins in heat-treated HPC-4 cells is correlated with increased resistance to LAK-mediated lysis.

@article{Vendetti2000InductionAM,
  title={Induction and membrane expression of heat shock proteins in heat-treated HPC-4 cells is correlated with increased resistance to LAK-mediated lysis.},
  author={Silvia Vendetti and Rosella Cicconi and Pierluca Piselli and Daniela Vismara and M{\'e}lanie Cassol and Andrea Delpino},
  journal={Journal of experimental & clinical cancer research : CR},
  year={2000},
  volume={19 3},
  pages={329-34}
}
In human pancreatic carcinoma cells (HPC-4), a hyperthermic treatment at 43 degrees C for 30 min resulted in the vigorous induction of Hsp72, along with a less pronounced increase in the rate of synthesis of Hsp90, Hsp60 and Hsp 27. Biotinylation of surface-exposed proteins, followed by isolation of biotin-tagged proteins by affinity chromatography, demonstrated that both Hsp72 and Hsp60 are expressed on plasma membrane. Membrane expression of these two Hsps was confirmed by immunoprecipitation… CONTINUE READING

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