Induction and inhibition of the allantoin permease in Saccharomyces cerevisiae

  title={Induction and inhibition of the allantoin permease in Saccharomyces cerevisiae},
  author={Roberta A. Sumrada and C A Zacharski and Vanessa Turoscy and Terrance G. Cooper},
  journal={Journal of Bacteriology},
  pages={498 - 510}
Allantoin uptake in Saccharomyces cerevisiae is mediated by an energy-dependent, low-Km, active transport system. However, there is at present little information concerning its regulation. In view of this, we investigated the control of alloantoin transport and found that it was regulated quite differently from the other pathway components. Preincubation of appropriate mutant cultures with purified allantoate (commercial preparations contain 17% allantoin), urea, or oxalurate did not… 
Allantoate transport in Saccharomyces cerevisiae
It is found that, contrary to earlier reports, the reaction catalyzed by allantoinase is freely reversible and exchange of intra- and extracellular allantoate was found to occur very slowly.
Allantoin transport in Saccharomyces cerevisiae is regulated by two induction systems
Induction is probably a transcriptionally regulated process, because addition of hydantoin acetate or oxalurate to the culture medium increased the steady-state levels of mRNA encoded by a gene required for allantoin transport (DAL4).
Regulation of allantoate transport in wild-type and mutant strains of Saccharomyces cerevisiae
The pleiotropic behavior of the dal4 and dal5 mutations are interpreted as deriving from a functional interaction between elements of the two transport systems, which appear to be sensitive to nitrogen catabolite repression, feedback inhibition, and trans-inhibition.
Genes of Different Catabolic Pathways Are Coordinately Regulated by Dal81 in Saccharomyces cerevisiae
Dal81, a general positive regulator of genes involved in nitrogen utilization related to the metabolisms of GABA, leucine, and allantoin, plays a central role in this coordinated regulation.
The allantoin and uracil permease gene sequences of Saccharomyces cerevisiae are nearly identical
The finding of these homologous sequences predicted to exist on the basis of DAL4's expression characteristics, supports and strengthens the suggestion that these elements mediate the functions the authors have previously ascribed to them.
Oxalurate transport in Saccharomyces cerevisiae
Observations suggest that failure of oxalurate to bring about induction of allophanate hydrolase in cultures growing under repressive conditions does not result from inducer exclusion, but rather from repression of dur1,2 gene expression.
The regulation of yeast gene expression by multiple control elements.
The purpose is the development of a model in the simple eucaryotic microorganism, Saccharomyces cerevisiae, of the metabolism of two main nitrogen sources, allantoin and arginine, and the selection of two systems instead of one to gain some insight into the spectrum of regulatory mechanisms that operate in eucarian cells.
Nitrogen catabolite repression in Saccharomyces cerevisiae
All known promoter sequences related to expression of nitrogen catabolite pathways are discussed as well as other regulatory proteins.


Allantoin transport in Saccharomyces cerevisiae
Allantoin uptake in both growing and resting cultures of Saccharomyces cerevisiae occurs by a low-Km transport system that uses energy that is likely generated in the cytoplasm, based on the observation that transport did not occur in the absence of glucose or the presence of dinitrophenol, carbonyl cyanide-m-chloro-phenyl hydrazine, fluoride, or arsenate ions.
Induction of the allantoin degradative enzymes in Saccharomyces cerevisiae by the last intermediate of the pathway.
  • T. Cooper, R. Lawther
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1973
It is shown that allophanic acid is required for synthesis of all enzymes participating in allantoin degradation, based upon the observation that wild-type strains produced a large amount ofallantoinase upon addition of allantoine, allantoate, ureidoglycolate, or urea to the medium.
Metabolite compartmentation in Saccharomyces cerevisiae
Uninduced cultures of Saccharomyces cerevisiae exhibit high basal levels of allantoinase, allantoicase, and ureidoglycolate hydrolase, the enzymes responsible for degrading allantoIn to urea, which suggests that at times metabolite compartmentation may play an equal role with enzyme induction in the regulation ofallantoin metabolism.
Nitrogen Repression of the Allantoin Degradative Enzymes in Saccharomyces cerevisiae
It is concluded that repression previously attributed to ammonia may result from its metabolism to amino acids and other metabolites.
Ureidosuccinic acid permeation in Saccharomyces cerevisiae.
The induction of urea carboxylase and allophanate hydrolase in Saccharomyces cerevisiae.
Regulatory Aspects of l-Glutamate Transport in Aspergillus nidulans
Wild-type cells of Aspergillus nidulans synthesize a transport system which appears to be specific for l-glutamate and l-aspartate, but the activity of this transport system is specifically impaired in a mutant at the aauA locus, which results in poor growth with l- glutamate or l- aspartate as the sole carbon or nitrogen source.
Mechanism of benzoic acid uptake by Saccharomyces cerevisiae.
  • B. Macris
  • Biology, Chemistry
    Applied microbiology
  • 1975
The evidence presented in this paper indicates that compounds of protein nature are involved in the uptake of this preservative, and the pattern of irreversible heat inactivation of the uptake system resembling protein denaturation by heat is evidenced.
Methylamine and ammonia transport in Saccharomyces cerevisiae
Methylamine (methylammonium ion) entered Saccharomyces cerevisiae X2180-A by means of a specific active transport system and was a strong competitive inhibitor of methylamine uptake, whereas other amines inhibited to a much lesser extent.
Characterization of cytosine permeation in Saccharomyces cerevisiae
The results support the hypothesis of a carrier-mediated transport, with reduced internal affinity, allowing the release and accumulation of cytosine in the inner compartment, and show first order kinetics.