Induction and in vitro culture of soybean crown gall tumors

  title={Induction and in vitro culture of soybean crown gall tumors},
  author={Hans Christian Pedersen and Jette Christiansen and R. Wyndaele},
  journal={Plant Cell Reports},
Induction of crown galls on 4–6 week old soybean (Glycine max (L.) Merr.) plants cultured in growth chambers was obtained with Agrobacterium tumefaciens strains C58, T37 and ACH5. The crown galls were isolated and cultured in vitro as sterile callus and liquid suspension cultures. Transformation was tested by opine tests and by molecular hybridization of restricted cell DNA with T-DNA fragments. Protoplasts were isolated from suspension cultures. Transformed protoplasts regenerate cell walls… CONTINUE READING
13 Citations
3 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 13 extracted citations


Publications referenced by this paper.
Showing 1-3 of 3 references

Gur l i t z RHG

  • AG Matthysse
  • Physiol Plant Pathol
  • 1982
1 Excerpt

V i l l a r roe l R

  • V l i e t F Van
  • Proc R Soc London
  • 1979

Plant Sci Lett I : 451456

  • WR Scowcroft, MR Davey, JB Power
  • Proc R Soc London Ser B
  • 1973

Similar Papers

Loading similar papers…