Induction and characterization of acrosome reaction in equine spermatozoa.

@article{Varner1987InductionAC,
  title={Induction and characterization of acrosome reaction in equine spermatozoa.},
  author={Dickson D Varner and C. R. Ward and Bayard Thayer Storey and Robert M. Kenney},
  journal={American journal of veterinary research},
  year={1987},
  volume={48 9},
  pages={
          1383-9
        }
}
Equine spermatozoa were incubated in a chemically defined medium for 8 hours. The medium preserved spermatozoal viability, as assessed by total spermatozoal motility, progressive spermatozoal motility, and spermatozoal exclusion of eosin stain. Effects of time and divalent cation ionophore, A23187, on the occurrence and character of the spermatozoal acrosome reaction were determined. Two light microscopic assays, a triple-stain technique and a chlortetracycline fluorescence assay, were… CONTINUE READING

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