Induction and Purification of C. difficile Phage Tail-Like Particles.

@article{Hegarty2016InductionAP,
  title={Induction and Purification of C. difficile Phage Tail-Like Particles.},
  author={J. Hegarty and William Sangster and Robert E. Ashley and Roland L. Myers and Susan L. Hafenstein and David B. Stewart},
  journal={Methods in molecular biology},
  year={2016},
  volume={1476},
  pages={
          167-75
        }
}
Due to the inherent limitations of conventional antibiotics for the treatment of C. difficile infection (CDI), there is a growing interest in the development of alternative treatment strategies. Both bacteriophages and R-type bacteriocins, also known as phage tail-like particles (PTLPs), show promise as potential antibacterial alternatives for treating CDI. Similar to bacteriophages, but lacking a viral capsid and genome, PTLPs remain capable of killing target bacteria. Here we describe our… 
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Clostridioides difficile Phage Biology and Application.
TLDR
The diverse array of bacteriophages (phages) that infect C. difficile could offer new treatment strategies and greater insight into the biology of the pathogen, and the most promising strategies for further development are highlighted.
Crystal structure of the R-type bacteriocin tube protein CD1364 from Clostridium difficile in the pre-assembled state
TLDR
Comparison of CD1363 and CD1364 with structures of PTLBs and related assemblies suggests that several conformational changes are required to form complete assemblies, and a preliminary model of the sheath and tube assembly from R-type diffocin is constructed.
Crystal Structures of R-Type Bacteriocin Sheath and Tube Proteins CD1363 and CD1364 From Clostridium difficile in the Pre-assembled State
TLDR
Comparison of CD1363 and CD1364 with structures of PTLBs and related assemblies suggests that several conformational changes are required to form complete assemblies, and a preliminary model of the sheath and tube assembly from R-type diffocin is constructed.
Isolation and characterization of Clostridium perfringens bacteriophages and optimization of electro-transformation parameters for Clostridium difficile
TLDR
It is suggested that the manuscript should be rewritten in a chapters-by- chapters format to facilitate more detailed discussion of the author's research and its intended uses.

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