Induction, purification and characterisation of acyl-ACP thioesterase from developing seeds of oil seed rape (Brassica napus)

@article{Hellyer1992InductionPA,
  title={Induction, purification and characterisation of acyl-ACP thioesterase from developing seeds of oil seed rape (Brassica napus)},
  author={Amanda Hellyer and Peter Francis Leadlay and Antoni R. Slabas},
  journal={Plant Molecular Biology},
  year={1992},
  volume={20},
  pages={763-780}
}
The level of two thioesterases, acyl-CoA thioesterase and acyl-ACP thioesterase was determined during seed maturation in oil seed rape. Both thioesterase activities rose markedly prior to the onset of lipid accumulation, but the induction kinetics suggest that the activities reside on distinct polypeptides. Acyl-ACP thioesterase (EC 3.1.2.14) was purified 2000-fold using a combination of ion exchange, ACP-affinity chromatogr aphy, chromatofocusing and gel filtration. Using native gel… CONTINUE READING

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