Inducible virus-mediated expression of a foreign protein in suspension-cultured plant cells

  title={Inducible virus-mediated expression of a foreign protein in suspension-cultured plant cells},
  author={K. Dohi and M. Nishikiori and A. Tamai and M. Ishikawa and T. Meshi and M. Mori},
  journal={Archives of Virology},
  • K. Dohi, M. Nishikiori, +3 authors M. Mori
  • Published 2005
  • Biology, Medicine
  • Archives of Virology
  • Summary.Although suspension-cultured plant cells have many potential merits as sources of useful proteins, the lack of an efficient expression system has prevented using this approach. In this study, we established an inducible tomato mosaic virus (ToMV) infection system in tobacco BY-2 suspension-cultured cells to inducibly and efficiently produce a foreign protein. In this system, a modified ToMV encoding a foreign protein as replacement of the coat protein is expressed from stably… CONTINUE READING
    Viral vectors for the expression of proteins in plants.
    • 338
    • PDF
    Viral vectors for production of recombinant proteins in plants
    • 186
    Plant viral vectors for delivery by Agrobacterium.
    • 103
    Estrogen-inducible GFP expression patterns in rice (Oryza sativa L.)
    • 24
    • PDF


    Publications referenced by this paper.
    Tomato Mosaic Virus Replication Protein Suppresses Virus-Targeted Posttranscriptional Gene Silencing
    • 200
    • PDF
    Geminivirus vectors for high-level expression of foreign proteins in plant cells.
    • 99
    Tobacco BY-2 Cell Line as the “HeLa” Cell in the Cell Biology of Higher Plants
    • 1,103
    • Highly Influential
    Plant cell cultures for the production of recombinant proteins
    • 485
    • PDF
    Inducible high-level mRNA amplification system by viral replicase in transgenic plants.
    • 43
    Tobacco mosaic virus and the virescence of biotechnology.
    • 60
    • PDF
    In vivo DNA expression of functional brome mosaic virus RNA replicons in Saccharomyces cerevisiae.
    • 92
    • PDF