Inducible isopentenyl transferase as a high-efficiency marker for plant transformation

@article{Kunkel1999InducibleIT,
  title={Inducible isopentenyl transferase as a high-efficiency marker for plant transformation},
  author={Tim Kunkel and Q W Niu and Y. Chan and Nam-Hai Chua},
  journal={Nature Biotechnology},
  year={1999},
  volume={17},
  pages={916-919}
}
Overexpression of the isopentenyltransferase gene (ipt) from the Ti-plasmid of Agrobacterium tumefaciens increases cytokinin levels, leading to generation of shoots from transformed plant cells. When combined with a dexamethasone-inducible system for controlling expression, ipt expression can be used to select for transgenic regenerants without using an antibiotic-resistance marker. The combined system allows efficient cointroduction of multiple genes (in addition to ipt) and produces… CONTINUE READING

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