Inducible expression of coding and inhibitory RNAs from retargetable genomic loci

@inproceedings{Weidenfeld2009InducibleEO,
  title={Inducible expression of coding and inhibitory RNAs from retargetable genomic loci},
  author={Ina Weidenfeld and Manfred Gossen and Rainer Loew and David Kentner and Stefan Perkmann Berger and Dirk G{\"o}rlich and Dusan Bartsch and Hermann Bujard and K. H. Sch{\"o}nig},
  booktitle={Nucleic acids research},
  year={2009}
}
Conditional gene expression systems have developed into essential tools for the study of gene functions. However, their utility is often limited by the difficulty of identifying clonal cell lines, in which transgene control can be realized to its full potential. Here, we describe HeLa cell lines, in which we have identified-by functional analysis-genomic loci, from which the expression of transgenes can be tightly controlled via tetracycline-regulated expression. These loci can be re-targeted… CONTINUE READING