Inducible expression of Tau repeat domain in cell models of tauopathy: aggregation is toxic to cells but can be reversed by inhibitor drugs.

@article{Khlistunova2006InducibleEO,
  title={Inducible expression of Tau repeat domain in cell models of tauopathy: aggregation is toxic to cells but can be reversed by inhibitor drugs.},
  author={Inna Khlistunova and Jacek Biernat and Yipeng Wang and Marcus Pickhardt and Martin von Bergen and Zuzana Gazova and Eckhard Mandelkow and E. -M. Mandelkow},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 2},
  pages={1205-14}
}
We generated several cell models of tauopathy in order to study the mechanisms of neurodegeneration in diseases involving abnormal changes of tau protein. N2a neuroblastoma cell lines were created that inducibly express different variants of the repeat domain of tau (tau(RD)) when exposed to doxycycline (Tet-On system). The following three constructs were chosen: (i) the repeat domain of tau that coincides with the core of Alzheimer paired helical filaments; (ii) the repeat domain with the… CONTINUE READING