Inducible cassette exchange: a rapid and efficient system enabling conditional gene expression in embryonic stem and primary cells.

@article{Iacovino2011InducibleCE,
  title={Inducible cassette exchange: a rapid and efficient system enabling conditional gene expression in embryonic stem and primary cells.},
  author={Michelina Iacovino and Darko Bosnakovski and Holger Fey and Danielle Rux and Gagan Bajwa and Elisabeth Mahen and Ana Mitanoska and Zhaohui Xu and Michael Kyba},
  journal={Stem cells},
  year={2011},
  volume={29 10},
  pages={1580-8}
}
Genetic modification is critically enabling for studies addressing specification and maintenance of cell fate; however, methods for engineering modifications are inefficient. We demonstrate a rapid and efficient recombination system in which an inducible, floxed cre allele replaces itself with an incoming transgene. We target this inducible cassette exchange (ICE) allele to the (HPRT) locus and demonstrate recombination in murine embryonic stem cells (ESCs) and primary cells from derivative ICE… CONTINUE READING
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