Inducement of a heat-shock requirement for germination and production of increased heat resistance in Bacillus fastidiosus spores by manganous ions.

@article{Aoki1973InducementOA,
  title={Inducement of a heat-shock requirement for germination and production of increased heat resistance in Bacillus fastidiosus spores by manganous ions.},
  author={Hiroyuki Aoki and Ralph A. Slepecky},
  journal={Journal of bacteriology},
  year={1973},
  volume={114 1},
  pages={137-43}
}
Bacillus fastidiosus, which requires uric acid or allantoin, grows and sporulates on a simple medium containing 59.5 mM uric acid, 5.7 mM K(2)HPO(4), and 2% agar in distilled water. Seventy to ninety percent sporulation was achieved in 96 h. Spores obtained on this medium do not need a heat shock prior to germination. The necessary germination conditions for this organism are 30 C, phosphate or this(hydroxymethyl)aminomethane buffer at pH 7.0, and 5.95 mM uric acid. Sporulation occurred earlier… CONTINUE READING
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